Determination Number Of Magnesium Nutritions From MgSO4.7H2O AND IRON FROM FeSO4.7H2O on Tetraselmiss chuii Cultivation To The Maximum Lipid Contact
DOI:
https://doi.org/10.35450/jip.v7i1.121Keywords:
FeSO4.7H2O, MgSO4.7H2O, lipid maximum Tetraselmis chuiiAbstract
This study discusses the effect of adding magnesium nutrients from MgSO4.7H2O and iron from FeSO4.7H2O to obtain maximum lipid levels in Tetraselmis chuii microalgae. The nutritional variations used were 0, 4, 6, 8 gr / L MgSO4.7H2O and 0, 24, 30, and 36 µM / L FeSO4.7H2O. Microalgae was cultivated with a photobioreactor filled with 1 L microalgae culture with a ratio of microalgae and sea water 3: 7, namely 300 ml of microalgae and 700 ml of sea water with salinity of 30 ppt. The light intensity is 2000 lux. The study began with culturing microalgae with variations in these nutrients. Then, the cell density is observed every 3 hours until the optimum time is obtained where the cell density is high. Then the microalgae is harvested and extracted so that the lipids are obtained. The extraction process was carried out using diethyl ether solvents. After obtaining the lipid mass, the microalgae lipid levels were calculated. The results showed that the highest lipid levels in Tetraselmis chuii microalgae were obtained by the addition of MgSO4.7H2O 6 gram and the addition of FeSO4.7H2O 36 µM which was 20,175%.
Downloads
References
Allen, JF, de Paula, BMW. Puthyiyaveetil, S. and Nield, J. 2011. Review: Structural Phylogenetic Map for Chloroplast Photosynthesis. Trends in Plant Science. 16 (12): 645-655.
Badan Pengkajian dan Penerapan Teknologi (BPPT). 2013. Outlook Energi Indonesia. Diakses dari www.bppt.go.id (waktu akses 4 November 2016 pukul 17.01 WIB).
Banerjee, A., Sharma, R., Chisty, Y., and Banerjee, U.C. 2002. Botryococcus braunii: A renewable source of hydrocarbons and other chemicals.
Critical Reviews in Biotechnology. (22) 3: 245–279. Bougis. 1997. Dalam Isnansetyo, A. dan Kurniastuty. 1995. Teknik Kultur Fitoplankton dan Zooplankton : Pakan Alami untuk Pembenihan Organisme Laut, Kanisius: Yogyakarta.
Brown, E. E. and J. B. Gratzek. 1980.Fish Farming Hand Book. Van Nostrand Reinhold Company. New York: 391 pp.
Butcher, R.W. (1959). An Introductory Account of the Smaller Algae of the British Coastal Waters. Part 1: Introduction and Chlorophyceae. Fishery Investigations London. Series IV, 1-74.
Chisti, Y.2007.“Biodiesel from Microalgae”. BiotechnologyAdvances, Vol.25, pp. 294-306.
El-metwally, A.E. Abdalla, F.E., El Saady, A.M., Safina, S.A., and El-Sawy S.S., 2010, Response of Wheat to Magnesium and Copper Foliar Feeding under Sandy Soil Condition, J. Am. Sci., 6 (12): 818-823.
Fabregas, Jaime., dkk. 1984. Growth of Marine Microalga Tetraselmis svecica in Batch Culture with Different Salinities and Concentration. Publisher. B.V. Amsterdam.
Fajrin, Ahfi. 2012. Pembuatan Biodisel dari CPO. https://www.scribd.com/doc/92543742/Pembuatan-Biodiesel-Dari-CPO. (diakses pada tanggal 29 November 2016, pukul 05.35 WIB).
FAO, 2009. Alga Based biofuels: a refiew of challenges and opportunities for developing countries (p. 49). Roma: Food and Agriculture Organisation of the United Nations.
Fitter A.H. dan Hay, R.K.M. 1991. Fisiologi Lingkungan Tanaman. Yokyakarta: Universitas Gajah Mada Press 1991
Fogg, G. E., Alga Cultures adan Phytoplankton Ecology, Medison, The Univercity of Wiconsin Press, 1987.
Gouveia, Luisa. 2011. Microalgae as a Feedstock for Biofuels. London: Springer Heidelberg Dordrecht.
Hoshida, H., Ohira, T., Minematsu, A., Akada, R., and Nishizawa, Y., (2005), Accumulation of Eicosapentaenoic Acid in Nannochloropsis sp. In Response to Elevated CO2 Concentrations, Applied Phycology, 17, pp. 29-34.
Ismi, Suko. 1996. Perkembangan Populasi Nannochloropsis oculata Pada Suhu dan Salinitas yang Berbeda. Jurnal Penelitian Perikanan Indonesia. II (2): 68-72.
Isnansetyo, A. dan Kurniastuty. 1995. Teknik Kultur Fitoplankton dan Zooplankton : Pakan Alami untuk Pembenihan Organisme Laut. Yogyakarta. Hal 14.
Kimball, J.W. 1983. Biologi. Diterjemahkan oleh Soetarmi. S. T. dan Sugiri, N. Jakarta: Gelora Aksara Pratama.
Kurniasih, Dora. 2014. Penambahan Nutrisi Magnesium Sulfat Mgso4.7H2O dan Nutrisi Kalsium Karbonat (Caco3) pada Kultifasi Tetraselmis chuii Untuk Mendapatkan Kandungan Lipid Maksimum. Prosiding Seminar Nasional Teknik Mesin. Universitas Trisakti. Jakarta (ID): EA02-1 – EA02-6.
Li Y, M. Horsman, N. Wu, C.Q Lan, and N. Dubois-Calero., 2008. Biofuels From Microalgae. Biotechnology Progress ; 24 (4) : 815–820
Lubian, L.M., Montero, O., Garrida, I.M., Hertas, I.E., Sobrino, C., Gonzales, M. and Pares, G. Nannochloropsis (Eustigmatophyceae) as a source of commercially valuable pigments. Journal of Applied Phycol., (12) 2000: 249-255.
Mujiman, Ahmad. 1984. Makanan Ikan. Cetakan 14. Penebar Swadaya. Jakarta. Prescott, G. W. 1978. How to Know The Freshwater Algae. Wne. Brown Company Publisher.
Nishio, J.N., J. Abadia and N. Terry. 1985. Chlorophyl Proteins and Electron Transport during Iron Nutrition Mediated Chlorophlast Development. Departement of Plant and Soil Biology. University of California. Barkeley. California.
Ratna Ningsih, Diah. 2013 Kadar Lipid Tiga Jenis Mikroalga pada Salinitas yang Berbeda. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung.
http://digilib.unila.ac.id/23574/3/SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf (Diakses pada tanggal 22 Desember 2017, pukul 12.01 WIB)
Richmond, Amos and Qiang, Hu. 2003. Handbook of Microalgal Culture. Wiley Blackwell: USA
Sasireka, G and Muthuvelayudham, R. 2015. Effect of Salinity and Iron Stressed on Growth and Lipid Accumulation In Skeletonema costatum for Biodiesel Production. India. Research Journal of Chemical Science. Vol.5(5), 69,72.
Steel, R.G.D. dan J.H. Torrie. 1993. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Terjemahan: M.Syah. Gramedia Pustaka Utama, Jakarta.
Sukardi, A. 2005. Anatomi Hewan. Bandung: Pustaka.
Sukmana, Wahyu. 2009. Salinitas. http://wahyusukmana.blogspot.co.id/2009/04/salinitas.html. (diakses pada tanggal 12 Desember 2016, pukul 06.07 WIB).
Sylvester B. D., D. Nelvy dan Sudjiharno, 2002. Dalam Seri Budidaya Laut No. 9.
Budidaya Fitoplankton dan Zooplankton. Balai Budidaya Laut Lampung. Direktorat Jenderal Perikanan Budidaya. Depertemen Kelautan dan Perikanan 24-36 hlm.
Taw. 1990. Petunjuk Kultur Murni dan Massal Mikroalga. UNDP. FAO. 1990.
Ugwu, C. U., Aoyagi, H. & Uchiyama, H. 2007. Influence of Irradiance, Dissolved Oxygen Concentration, and Temperature on the Growth of Chlorella sorokiniana. Photosynthetica, 2(45), pp. 309-311.
Wang, B., Li, Y., Wu, N., Lan, Q., C. 2008. CO2 Bio-Mitigation Using Microalgae. Applied Microbiology Bioetechnology. 79: 707-718.
Wilde, C. and Benemann, G. (1993).A Culture Method for Microalgae Forms to Studies on Growth and Carotenoid Production. World Journal of Microbiology and Biotechnology. Volume (17):325-329.
Yuharma, Devega. 2013. Biodisel. https://devegayuharma.wordpress.com/, (diakses tanggal 1 Desember 2016, pukul 06.10 WIB).
Published
How to Cite
Issue
Section
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shell be assigned to Development Innovation: Jurnal Kelitbangan (JIP) , Balitbangda Provinsi Lampung as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations, the reproduction of any part of this journal, its storeage in databases and its transmission by any form or media. such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc.
